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Alternatively, the stock culture organisms might be developed in an acceptable liquid medium (i.e., SoybeanCasein Digest Broth or Sabouraud Dextrose Broth) and the cells harvested by centrifugation, then washed and resuspended in sterile saline TS to get a microbial count of about 1 × 108 cfu for each mL. [NoticeThe estimate of inoculu